QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION
نویسندگان
چکیده
منابع مشابه
Quantitative Studies on Antibody Purification
1. Quantitative data are given on the effect of changes in hydrogen ion concentration and of salt solutions of high concentration on certain immune precipitates obtained at lower salt concentration. 2. Advantage is taken of the shift in reaction equilibrium brought about by the salt in the case of pneumococcus carbohydrate-anti-carbohydrate precipitates to enable the preparation, in a single st...
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Young adult mice were primed with 20 Lf (56 microg) of diphtheria toxoid and given a second injection of the same size 40 days later. This procedure produces a reproducible secondary response which can be used as a standard. Chloramphenicol in maximum dosage prevents the unknown process by which the animal is primed for the second response. To be fully inhibitory, the drug must be given from th...
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The objective of the present study was oriented to produce purified polyclonal antibody to prepare a prolactin solid phase coated tubes radioimmunoassay system. In the present study, production of polyclonal antibodies was carried out through immunization of three healthy white male mature New Zealand rabbits with a highly purified sheep prolactin antigen. The obtained anti-sera was purified us...
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A study of the kinetics of antibody-producing cells has been carried out by the use of rosette formation for detection of individual antibody-producing cells, and labeling with tritiated thymidine, in cells obtained from mouse spleens at intervals after injection of SRBC. Following a primary injection of the antigen, the number of RFC per million cells was found to increase to a peak at 5 days,...
متن کاملStudies on Non-precipitating Antibody
Reinjection of antigen into immunized rabbits usually results in an immediate increase of circulating antibody-antigen complex. This immediate formation of complex seems to depend upon the presence of non-precipitating antibody before reinjection. The complex disappears by the 2nd day after reinjection and reappears between the 4th to 7th day. This is earlier than its first appearance after a p...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1938
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.67.2.181